Ichabod doesn't seem to have had any. He only ever mentions his father in terms of what he gave up when he changed sides.
Giovanni has a twin sister, Francesca. She's a feminist ahead of her time, writes feminist tracts under a man's name, puts on a fake moustache to go to the university, and is generally awesome.
Athelstan had four brothers and a sister. They're all dead. That's really all we know about them.
Harry has a sister who lives in Ireland. She's a really nice woman and a wizard and while they're not super-close, he trusts her enough to send Nita to stay with her.
Nancy doesn't talk about her family. If she had siblings, she hasn't seen them since before she had Jamie.
Jonathan doesn't have blood siblings, but he has Andrew.
Michael Carpenter may have some, he's from a Catholic family, but they've never appeared or been mentioned.
Roshaun is an only child.
Norrington probably does - or did, they may have died in childhood given his time period - but canon never confirms either way.
Gavroche has Eponine in terms of blood siblings and is very confused by who this Azelma is that people keep asking about. He also considers all of Les Amis his brothers. And Ingress and the other HoA kids, of course.
None of my pups have siblings in the biological sense, oddly enough. Kreyu's "brothers" or broodmates aren't related to her. Which is a given since dragons don't reproduce.
Ibani has no memory of any biological family and Annabelle Newfield was an only child.
Brimstone doesn't even know or remember his parents, let alone any siblings. If he had them, they are long gone, like the rest of his tribe/clan.
Andrea didn't have any brothers or sisters but lots of cousins. They were all bullies and quite brutal. Note the past tense.
Lady Trent/Isabella has five brothers. She is the youngest child and the only daughter. Not much is mentioned of the oldest brothers (I imagine them as being proper Victorian-esk gentlemen) but the youngest, Andrew, is mentioned and he seems to be a forward thinking sort. At least he cares about Isabella's wants and dreams.
Quin is an only child, as well, and his parents died when he was young. He does have an extended family with many cousins (at least I imagine so) but he is pretty estranged from them.
Sam I have head-canonned as being an only child but he has at least one cousin that is more of a sister to him. She is very technical and mathematically inclined and grew to work for Stark Industries; being instrumental in building the falcon suit.
ETA: And Ethan, how did I forget Ethan? He does have a father that is mentioned in canon but no mention is made of any siblings. I have trouble believing in him being an only child though, given the time period, when having many children was nearly a duty.
Eriond has six living older brothers and one deceased brother. His living brother are: Aldur, the eldest, wise and gentle until pissed off; Mara, a little overly-interested in reproductive activities and was in a sheer depression over the destruction of his people until it turned out they were alive; Chaldan, the Bull God, not the brightest ever; Issa, the Snake God, who sleeps a lot; Nedra, the Merchant God who likes money; and Belar--who many of you know pretty well, I think. The dead brother was Torak, the Dragon God, who became a batshit crazy genocidal racist maniac trying to conquer the world. Also arguably Eriond's an adopted sibling of Polgara's twins and possibly the entire Rivan line.
Lois has a little sister, Lucy, who she half-raised despite being all of six when their mother died. Lucy is an international criminal for a while, nearly gets Lois killed more than once... they're complicated.
Tavi kind of had siblings in the form of his cousins he grew up with, Bernard and his first wife Cassea's two daughters, but genetically, he is very much an only child.
Well, canon says nothing explicit about Fairy Fixit having a family, but there is the Tooth Fairy/Fairy General/Fairy Tooth Hunter who has a suspiciously similar appearance, dress style, and manner of talking. Well, maybe I am reading too much into the fact that they are the only fairies that wear big poofy pants. But, anyhow, where Fairy Fixit is a nerd about teleportation equipment, the Tooth Fairy is a nerd about the military. The fairies don't even have a proper military, but she organized and lead the counter-revolution against the Fairy Mafia anyway :P.
Amascut's siblings. Oh boy. Well they are generally a well liked bunch but their relationship with Amascut is severely strained. Her closest sibling and blood brother is Icthlarin who has been the sole god of death since Amascut left the job millenia ago. He has a very strong sense of responsibility for the dead and for his sister, whom he wishes greatly would recover. He is generally a stand up guy and doesn't try to carve out a kingdom or empire for himself. His duty is to the dead and getting them to the afterlife safely; he doesn't have much time for other things.
Then there are her adopted siblings, who her father raised to godhood. Apmeken was raised from a monkey as the goddess of sociability and social pleasures. She was the trickster goddess of the group, until Amascut stole her voice, hearing, and sight. She is a lot more somber now, and by the time canon comes around and she recovers her senses, she becomes more focused on stopping Amascut. Apmeken has three natures; that of the skillfull and acrobatic monkey, that of the wise ape, and that of the comical baboon. Scabaras was raised from a scarab beetle to be the god of isolation and contemplation. Most modern (by canon time) religious texts say that he was ascended as a counter example and warning of how people shouldn't act, but he is sometimes viewed positively as the god of scholarship and asceticism. He has been shy and not shown himself in canon, though Amascut does accuse him of trying to stop her via dung beetles. I am guessing he is trying to get back at her for tricking him and his followers into doing something very bad which earned him his current bad reputation. Then there is Crondis who was raised from a crocodile to be goddess of temperance and physical pleasures. Um... canon hasn't dealt with her much except to say that the rich hate her, since she is a party pooper and a goddess of party poopers and is very against storing up of wealth for wealth's sake. And finally, Het, raised from a human to be god of physical strength and health. He is generally well loved by everyone, especially the people of Al-Kharid, since he is their patron god of sorts. Well, the people of Sophanem might be going "WHY HAVE YOU ABANDONED US??!!" at him by canon time.
Gordon Freeman has an older brother, Jay. Jay was always more athletic and competitive than Gordon, but he died sometime after the Black Mesa incident.
Adrian Shephard had four siblings; his formerly younger brother and his older sister are the only ones left alive. Dana, the sister, was originally an elementary school music teacher. These days she's got a lot of curriculum preparation to do in anticipation of when the children start being old enough for school again. Dan, the brother, had been lined up to go to college on scholarship money from a timber company when the Combine invaded. He had to fade back into the Rowlesburg survivor community and stay out of sight; he's been a messenger/courier for some time and does carpentry these days, and spares no opportunity to make fun of his formerly older brother. (Adrian was caught out of time by the G-Man for twenty years; Dan was a year or so younger than Adrian when it happened, so now he's the older sibling.)
Stacker Pentecost had one sister, Luna, who tended to be more idealistic and a little more hotheaded; she wanted to 'kill a dragon', according to dialogue in Tales From year Zero. She died battling the Trespasser.
Varric has one older brother, Bartrand, who's a jerk even when he's not handling [SPOILER SPOILER SPOILER]. Varric puts up with him, though.
Medic has a number of younger siblings, I think, but he doesn't talk about them much. I get the feeling it's two sisters. More than that I don't know.
Neither Edward Kenway nor Santo have siblings.
Ellen believed she was an only child for the first nineteen years of her life. She has not trusted her late lamented father's word in some time, however, and privately wonders sometimes if Dad didn't leave behind a couple of Park bastards somewhere along the way to the Capital Wastes.
Enjolras is canonically an only child. Depending on mood and context, he might very easily call the other Amis his brothers (and has in-bar, a few times) or all the fighters at the barricades, or even call all the people of France his brothers (and... maybe remember to mention the sisters), but that's a little different.
Cosette is also an only child, both biologically and for how she was raised.
Thor: AHAHAHAHAHAHAHAHA *facedesk*. Uh, yes! Yes, he has a brother! Loki and Thor are very alike in some ways, and very very different in several others. They used to be very close, but nowadays their relationship is, uh. Let's say tumultuous.
Trowa grew up as a war orphan without siblings, but he and his sister Catherine adopted each other as teenagers. Cathy is cheerful and nurturing and extremely stubborn, and she and Trowa both trade off being the protective 'older' sibling depending on the context. They're very close. (She's actually his biological sister too -- they were separated as toddlers -- because anime plotlines, but neither of them knows that, so it doesn't affect anything ICly.)
Clare may or may not have originally had any siblings as a kid, but her whole family is dead, so uh. Nor really any adoptive ones; she doesn't look at the other Claymores that way, and I don't think Teresa counts. Or Raki.
River, like Thor, has a brother whom she loves very much! Unlike Thor, they remain close, with absolutely zero attempted fratricide. Simon and River have settled into being adult friends nowadays, and Simon doesn't live on Serenity anymore so they talk by waves or at Milliways more often than with visits, but they'll always be close.
Sonya Blade: Sonya's game bio in the official Mortal Kombat comic reads that she had a twin brother who's deceased. Given that there's no rank by his name, he most likely died at a young age. No other information is given, but I have it that Sonya and her brother were close, given that they were army brats and had to move around a lot.
Matilda Wormwood. Matilda has an older brother named Mike. Unlike her parents, Matilda and mike seem to get along fairly well, at the very least he doesn't tease her and lets her be. She's also never directed a prank at him or thought bad of him either.
Also noted, when her parents leave for Spain at the end of the book, Mike's the only one who waves back at her through the car.
Claudia: Joshua. She nearly died for him once, and would do it again in a heartbeat.
Apollo: Only one full sibling; he and Artemis don't always get along, but harm her at your own peril. She'll fuck you up first and he'll be right behind her. He also has a hojillion half-siblings (thanks, Dad), a lot of whom he doesn't know all that well. Thalia's a good kid, though.
Imp: A few older brothers. They're more into the family druid business than he ever was.
Regulus: AHAHAHAHAHAHAHAHAHA welp. He and Sirius used to get along like gangbusters! And then Gryffindor happened, and they're only just - tentatively, and VERY VERY CAREFULLY - starting to patch things up after five years of not really talking to each other.
Red: If she does, she's never met them. But given that she didn't know her mother was still alive for a long time and never met her father, I can't rule out full or half siblings her grandmother didn't find out about in time to raise.
Woolly: Is the fourth child and third/youngest son of five. His oldest brother is worthless; his older sister married for money; his other brother's a rabbi; and he was always closest to his younger sister. She was the only one to find out he did not in fact die in 1901.
Clementine: Has a bunch of brothers, all of whom used to be protective of her. Now they all have their own lives, and she misses them.
Dixie: Has an (adopted - that's my headcanon and the only thing that fits canon entirely) sister, Dolly, who owns a casino in Reno and is Dixie's rival. She has a penchant for trying to steal anything Dix gets too attached to, including Brisco.
Juliet: Has two brothers; in the show we only meet Ewan, who is slightly older than her and is a military man and her idol - the inspiration for her being a cop. Things don't go well for Jules when she finds out Ewan might be doing some very illegal things; he's currently on the run.
Pinkie: Has two younger sisters, Marble and Limestone, and an older sister, Maud. Pinkie is very close to Maud, even though they have little in common.
Eponine: Has a baby brother, Gavroche, whom she dotes on. She's also aware that Azelma exists, though, like Gavroche, she didn't have the pleasure of knowing her in life.
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Giovanni has a twin sister, Francesca. She's a feminist ahead of her time, writes feminist tracts under a man's name, puts on a fake moustache to go to the university, and is generally awesome.
Athelstan had four brothers and a sister. They're all dead. That's really all we know about them.
Harry has a sister who lives in Ireland. She's a really nice woman and a wizard and while they're not super-close, he trusts her enough to send Nita to stay with her.
Nancy doesn't talk about her family. If she had siblings, she hasn't seen them since before she had Jamie.
Jonathan doesn't have blood siblings, but he has Andrew.
Michael Carpenter may have some, he's from a Catholic family, but they've never appeared or been mentioned.
Roshaun is an only child.
Norrington probably does - or did, they may have died in childhood given his time period - but canon never confirms either way.
Gavroche has Eponine in terms of blood siblings and is very confused by who this Azelma is that people keep asking about. He also considers all of Les Amis his brothers. And Ingress and the other HoA kids, of course.
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Ibani has no memory of any biological family and Annabelle Newfield was an only child.
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Brimstone doesn't even know or remember his parents, let alone any siblings. If he had them, they are long gone, like the rest of his tribe/clan.
Andrea didn't have any brothers or sisters but lots of cousins. They were all bullies and quite brutal. Note the past tense.
Lady Trent/Isabella has five brothers. She is the youngest child and the only daughter. Not much is mentioned of the oldest brothers (I imagine them as being proper Victorian-esk gentlemen) but the youngest, Andrew, is mentioned and he seems to be a forward thinking sort. At least he cares about Isabella's wants and dreams.
Quin is an only child, as well, and his parents died when he was young. He does have an extended family with many cousins (at least I imagine so) but he is pretty estranged from them.
Sam I have head-canonned as being an only child but he has at least one cousin that is more of a sister to him. She is very technical and mathematically inclined and grew to work for Stark Industries; being instrumental in building the falcon suit.
ETA: And Ethan, how did I forget Ethan? He does have a father that is mentioned in canon but no mention is made of any siblings. I have trouble believing in him being an only child though, given the time period, when having many children was nearly a duty.
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Eriond has six living older brothers and one deceased brother. His living brother are: Aldur, the eldest, wise and gentle until pissed off; Mara, a little overly-interested in reproductive activities and was in a sheer depression over the destruction of his people until it turned out they were alive; Chaldan, the Bull God, not the brightest ever; Issa, the Snake God, who sleeps a lot; Nedra, the Merchant God who likes money; and Belar--who many of you know pretty well, I think. The dead brother was Torak, the Dragon God, who became a batshit crazy genocidal racist maniac trying to conquer the world. Also arguably Eriond's an adopted sibling of Polgara's twins and possibly the entire Rivan line.
Lois has a little sister, Lucy, who she half-raised despite being all of six when their mother died. Lucy is an international criminal for a while, nearly gets Lois killed more than once... they're complicated.
Tavi kind of had siblings in the form of his cousins he grew up with, Bernard and his first wife Cassea's two daughters, but genetically, he is very much an only child.
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Amascut's siblings. Oh boy. Well they are generally a well liked bunch but their relationship with Amascut is severely strained. Her closest sibling and blood brother is Icthlarin who has been the sole god of death since Amascut left the job millenia ago. He has a very strong sense of responsibility for the dead and for his sister, whom he wishes greatly would recover. He is generally a stand up guy and doesn't try to carve out a kingdom or empire for himself. His duty is to the dead and getting them to the afterlife safely; he doesn't have much time for other things.
Then there are her adopted siblings, who her father raised to godhood. Apmeken was raised from a monkey as the goddess of sociability and social pleasures. She was the trickster goddess of the group, until Amascut stole her voice, hearing, and sight. She is a lot more somber now, and by the time canon comes around and she recovers her senses, she becomes more focused on stopping Amascut. Apmeken has three natures; that of the skillfull and acrobatic monkey, that of the wise ape, and that of the comical baboon. Scabaras was raised from a scarab beetle to be the god of isolation and contemplation. Most modern (by canon time) religious texts say that he was ascended as a counter example and warning of how people shouldn't act, but he is sometimes viewed positively as the god of scholarship and asceticism. He has been shy and not shown himself in canon, though Amascut does accuse him of trying to stop her via dung beetles. I am guessing he is trying to get back at her for tricking him and his followers into doing something very bad which earned him his current bad reputation. Then there is Crondis who was raised from a crocodile to be goddess of temperance and physical pleasures. Um... canon hasn't dealt with her much except to say that the rich hate her, since she is a party pooper and a goddess of party poopers and is very against storing up of wealth for wealth's sake. And finally, Het, raised from a human to be god of physical strength and health. He is generally well loved by everyone, especially the people of Al-Kharid, since he is their patron god of sorts. Well, the people of Sophanem might be going "WHY HAVE YOU ABANDONED US??!!" at him by canon time.
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Adrian Shephard had four siblings; his formerly younger brother and his older sister are the only ones left alive. Dana, the sister, was originally an elementary school music teacher. These days she's got a lot of curriculum preparation to do in anticipation of when the children start being old enough for school again. Dan, the brother, had been lined up to go to college on scholarship money from a timber company when the Combine invaded. He had to fade back into the Rowlesburg survivor community and stay out of sight; he's been a messenger/courier for some time and does carpentry these days, and spares no opportunity to make fun of his formerly older brother. (Adrian was caught out of time by the G-Man for twenty years; Dan was a year or so younger than Adrian when it happened, so now he's the older sibling.)
Stacker Pentecost had one sister, Luna, who tended to be more idealistic and a little more hotheaded; she wanted to 'kill a dragon', according to dialogue in Tales From year Zero. She died battling the Trespasser.
Varric has one older brother, Bartrand, who's a jerk even when he's not handling [SPOILER SPOILER SPOILER]. Varric puts up with him, though.
Medic has a number of younger siblings, I think, but he doesn't talk about them much. I get the feeling it's two sisters. More than that I don't know.
Neither Edward Kenway nor Santo have siblings.
Ellen believed she was an only child for the first nineteen years of her life. She has not trusted her late lamented father's word in some time, however, and privately wonders sometimes if Dad didn't leave behind a couple of Park bastards somewhere along the way to the Capital Wastes.
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AHAHAHAHAHAHAHA
*sobbing*
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Cosette is also an only child, both biologically and for how she was raised.
Thor: AHAHAHAHAHAHAHAHA *facedesk*. Uh, yes! Yes, he has a brother! Loki and Thor are very alike in some ways, and very very different in several others. They used to be very close, but nowadays their relationship is, uh. Let's say tumultuous.
Trowa grew up as a war orphan without siblings, but he and his sister Catherine adopted each other as teenagers. Cathy is cheerful and nurturing and extremely stubborn, and she and Trowa both trade off being the protective 'older' sibling depending on the context. They're very close. (She's actually his biological sister too -- they were separated as toddlers -- because anime plotlines, but neither of them knows that, so it doesn't affect anything ICly.)
Clare may or may not have originally had any siblings as a kid, but her whole family is dead, so uh. Nor really any adoptive ones; she doesn't look at the other Claymores that way, and I don't think Teresa counts. Or Raki.
River, like Thor, has a brother whom she loves very much! Unlike Thor, they remain close, with absolutely zero attempted fratricide. Simon and River have settled into being adult friends nowadays, and Simon doesn't live on Serenity anymore so they talk by waves or at Milliways more often than with visits, but they'll always be close.
Regan is an only child.
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Matilda Wormwood. Matilda has an older brother named Mike. Unlike her parents, Matilda and mike seem to get along fairly well, at the very least he doesn't tease her and lets her be. She's also never directed a prank at him or thought bad of him either.
Also noted, when her parents leave for Spain at the end of the book, Mike's the only one who waves back at her through the car.
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Sam: Nope.
Claudia: Joshua. She nearly died for him once, and would do it again in a heartbeat.
Apollo: Only one full sibling; he and Artemis don't always get along, but harm her at your own peril. She'll fuck you up first and he'll be right behind her. He also has a hojillion half-siblings (thanks, Dad), a lot of whom he doesn't know all that well. Thalia's a good kid, though.
Imp: A few older brothers. They're more into the family druid business than he ever was.
Regulus: AHAHAHAHAHAHAHAHAHA welp. He and Sirius used to get along like gangbusters! And then Gryffindor happened, and they're only just - tentatively, and VERY VERY CAREFULLY - starting to patch things up after five years of not really talking to each other.
Red: If she does, she's never met them. But given that she didn't know her mother was still alive for a long time and never met her father, I can't rule out full or half siblings her grandmother didn't find out about in time to raise.
Woolly: Is the fourth child and third/youngest son of five. His oldest brother is worthless; his older sister married for money; his other brother's a rabbi; and he was always closest to his younger sister. She was the only one to find out he did not in fact die in 1901.
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Dixie: Has an (adopted - that's my headcanon and the only thing that fits canon entirely) sister, Dolly, who owns a casino in Reno and is Dixie's rival. She has a penchant for trying to steal anything Dix gets too attached to, including Brisco.
Juliet: Has two brothers; in the show we only meet Ewan, who is slightly older than her and is a military man and her idol - the inspiration for her being a cop. Things don't go well for Jules when she finds out Ewan might be doing some very illegal things; he's currently on the run.
Pinkie: Has two younger sisters, Marble and Limestone, and an older sister, Maud. Pinkie is very close to Maud, even though they have little in common.
Eponine: Has a baby brother, Gavroche, whom she dotes on. She's also aware that Azelma exists, though, like Gavroche, she didn't have the pleasure of knowing her in life.